LOCUS       40924_7481       10843 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBS[NBL KO 1], complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Davis RJ, Belikoff EJ, Scholl EH, Li F, Scott MJ.
  TITLE     no blokes Is Essential for Male Viability and X Chromosome Gene 
            Expression in the Australian Sheep Blowfly
  JOURNAL   Curr. Biol. (2018) In press
  PUBMED    29887311
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Davis RJ, Belikoff EJ, Scholl EH, Li F, Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2018) Entomology and Plant Pathology, North 
            Carolina State University, 112 Derieux Place, 1546 Thomas Hall, 
            Raleigh, NC 27607, USA
REFERENCE   3  (bases 1 to 10843)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. Biol.
            (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-APR-2018) Entomology and Plant Pathology, North Carolina State 
            University, 112 Derieux Place, 1546 Thomas Hall, Raleigh, NC 27607, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..1133)
                     /label=homology arm derived from Lucilia cuprina NBL
                     /note="homology arm derived from Lucilia cuprina NBL"
     promoter        complement(1164..1182)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1203..1219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1227..1243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1251..1281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1296..1317)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1605..2193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2367..3224)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3225..3329)
                     /label=AmpR promoter
     rep_origin      complement(3355..3810)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3952..3968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3978..3996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(4008..5073)
                     /label=homology arm derived from Lucilia cuprina NBL
                     /note="homology arm derived from Lucilia cuprina NBL"
     regulatory      5074..8495
                     /label=derived from Lucilia cuprina h83
                     /note="derived from Lucilia cuprina h83"
                     /regulatory_class="promoter"
     CDS             8496..9170
                     /label=DsRed-Express2
                     /note="noncytotoxic tetrameric variant of DsRed fluorescent
                     protein (Strack et al., 2008)"
     misc_feature    9174..10599
                     /note="derived from Lucilia cuprina atub UTR and
                     polyadenylation signal sequence"
     protein_bind    10663..10762
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 ggttctgcag catccaacat acgattgtag gcatcaatgg ccttctctac actttgatag
       61 cgtataaatg cataacgagt atatttcata cgttgtgcaa aaccaatatc aatacgtgca
      121 gcaccaggaa aatgattctt tagggtttta cacgctatag ttgttggcaa attgcccacg
      181 tacaaagtat aaggatcaac ttcattcaat ttagtactat ggactttata gggtatttgt
      241 ttaatttcca ctgataaaaa tcccgtacca aatggtacct tcgataggtc atcgatagtt
      301 ttctccacat ctgttcctgg tttcagatgt accaaacaat aacgtggcgc ataattcaat
      361 tgaaaatgca cagattctat atcaggacta taacatttta caatttcacg acttagttca
      421 ggatcaggaa aacgagtgat taatattata tctgtctgta gttttctggc atttgactct
      481 tcggccttaa gggccagtat ggcatcttct tcgcaccagg atttacttaa gcgatatcgt
      541 tccgaaatgg cttttcgttt tgttttcatt ttaacaattg ttttagccgc tgaaatggaa
      601 ataaaagtta tgagtcccaa gggaatagtt tttcttataa tgctgtctaa aagtgaagac
      661 cattttaatg gccctagttt agaaatatta aactaataat tttaatatct taccagtttt
      721 cttatcactt gatatattat ttttgaaatt aaattttgct gaacttggtg acgatgttgt
      781 aggagacata gaggaagcgg acattggtgg tggcggtaaa tatggcaatt tatcatcact
      841 tggcaatgga ggtagtatag gtggtggctg tggttgaggc agtggcggct gttgctgcag
      901 ctgatgttgt ggcaattttg gcggtggtgt agatgttatt gtttgccaca taaatggagt
      961 tatagcatca gtcacgccat atgatgaagt ggtatctgtc gaaactctgc cgttattata
     1021 actggtggga taataactat tataaccaaa tccatataaa ttagaattat ccatcatatt
     1081 gggagtgagc tgctgctgtt ggctattttg tgccgccact gaagttgagg gccgccaccg
     1141 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     1201 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2041 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     2701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     3361 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3421 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3481 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3541 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3601 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3661 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3721 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3781 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     3841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3901 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3961 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc actgtggctg
     4021 ccaaaacgtc ttcaggttgt ttatttcttt tagctggtgg cggtcctaaa ttttccacac
     4081 aattagcacg catctgagat ctctgacaat tttgtaaaaa ctctcctaaa ctatttgtcg
     4141 ataccgtaga catggtatct gtttcatcag ctgttgagtg taaagaaact acatcgttgt
     4201 tatttgactt tccggaattt tccttattat tatcattttg attattggga ggtgcaaaaa
     4261 attcatcata taatgctatt tgttcctcct gttttatatt atcattgttt ccatccaagc
     4321 gagaagttag agcattatcg cgtacaacta tatcagacgg atcaatatca ggttctggtt
     4381 ttatattagg gactattgag ttgctattgt ttgctgaaaa tgttaaagag atgtagatgt
     4441 agttttttaa aaactttaac ttaatagttt gtggaatacc ttgacttgtg ctgattgtag
     4501 gaggttcagt gttattggtt tccaatgctg aattgtctcc agttgaaaaa cttaactttg
     4561 ttttattgtg taaactctca attgtgcttg taattgaagt ggacgacgtt tagaggtata
     4621 aaaacattat cttctgcttc tgaatttttt tcagcagtgg aagcggcttc cgttgaatta
     4681 ttatctgtaa gaaagtaata ttcctaactt aaaataaagt ttctcaaaat gtttacttaa
     4741 tgttaaaata tttggggact ttgccagagg acagtataca aagttgtata agctacaagc
     4801 aacttaccag tacttatatc caaatcatgt gaaacttttt caacgccatc atttgagtta
     4861 tgttcctcaa catctgcagt attatttgcc tgtaaaacat caaatgattc ggtattttct
     4921 ccaacagttt tttcatctga ttctagtgtt gtgtgattca ttagttcatc atcatgcata
     4981 ctatcgagat gttcagcatc tccatcttta ttgccctcag ttgtagctgt aacacgtcta
     5041 aaacgtatag ttattgtacg accacctatt tggcgccctc ttttgtcctt agtttcatat
     5101 gttcctaata tctgaaaaat atacaattga taagaattac tgaatgttat taaatggata
     5161 aaattaaaca ttattgattg ttgttgatat aaagatttac ggaaaaatat attcagatac
     5221 aacttcaaat agaactagaa ctgaactaga actgaactag aactgaacta gaactgaact
     5281 aaaactgaac tagaactaaa ctagaactga actagaactg aactagaact gaactagaac
     5341 tgaactagaa ctgaactaga actaaactag aactgaacta gaaccgaact agaactgaac
     5401 tagaactgaa ctagaactga actagaactg aactagaact gaactagaac tgaactagaa
     5461 ctgaactaga actgaactag aactgaacta gaactgaact agaactgaac tagaactgaa
     5521 ctagaactga actagaactg aactagaact gaactagaac tgaactagaa ctgaactaga
     5581 actgaactag aactgaacta gaactgaact agaactgaac tagaactgaa ctagaactga
     5641 actagaactg aactagaact gaactagaac tgaactagaa ctgaactaga actgaactag
     5701 aactgaacta gaactgaact agaactgaac tagaactgaa ctagaactga actagaactg
     5761 aactagaact gaactagaac tgaactagaa ctgaactaga actgaactag aactgaacta
     5821 aaacagtact agaactcatc ccaaaccgaa ctaaagctaa aactggaata aattccttaa
     5881 aaaaatgtta gcggaaaaga atggttcctc tcgatgaagt gcagtcgtaa tcttccatag
     5941 atggaaccct tgtgcgataa gtaatcctgg tgcaactata cacaaaacaa agcaatcaat
     6001 atctacggct atgcataatc ccacattctc ccaacagaaa tatgaaagta gaagttctag
     6061 atttgtaaac ccaagccaaa accattttac ttataaaatc tttctttaaa tcacgaaaaa
     6121 gttaattaaa tctatgtatt tatcatttct ttaagaccta ccattggaat tatactaata
     6181 attttcagtt tcttaccttg gaatcataga gaacttttgc tttcgaagga tctggttcat
     6241 aacatagaac tttttcgccc ttctcgaaat aacgagaacg gggcccacgt gttgaaacca
     6301 ttatcagaaa ataattcact taaacattat aatttgataa aaagaacact aaaacaaaat
     6361 attataatta taccgccaaa aattcaacaa actttaagaa atttcgagta aattgcaccg
     6421 caaactaaaa aaccgacatg ataaacaaaa aaaatacata aaagagcgca aacagctgtc
     6481 atttacaggg ttgtagatat ttgaaatttt tacctaaaaa ggggaaaata aaaggccaca
     6541 cttttggaaa ttacaaattt gtgagagaat ttttgatttc atttattaaa tttatataaa
     6601 aacaaaagag ttgttataat atgtatgaga taatgtttgc aaattttaat ttttaaattt
     6661 ttacagtcaa attttataaa atatgttaaa agtatatgat tgtcacattt ttaaattatt
     6721 ccacttttca ttgtttttat tcaagaccgg caactctgat gttttgctgc acatcagctg
     6781 cactggcgaa aaaattatta atgcccccat gatgagagaa aatcaatatt ttttcatttt
     6841 atgcattttt ataaattttg ttagcaatat tattgtttta ataatgttat tattttcttt
     6901 aataaaacaa ttaaattact ttcatttata atttatgatg actaatatgt aaatcgaatg
     6961 tgattaaatc atacatacta tttccatata aagcacaaag tttaatatat aattaatatt
     7021 aaatttattt aatttaaaaa ttaatagtta atacatattt caattaatca aatacatatt
     7081 aagcttgtaa cttagttcaa aaaacactta attatacata gatttgtcaa ttaataagac
     7141 tttttaacta taatttaaag aataaaacgt ttttgaaata aataagttca ttgtttgact
     7201 agaaaaatat ttagtttatg tcatgatatt actcagcaat gcctaagatt aattcctttt
     7261 tcatttaatt aataactttt atatattttt gcataaataa ataaagtaaa ttatagttga
     7321 ttcattccgc atgttattaa aaacagagag aacattgtag aaattttgac attgatgcaa
     7381 ttttacagtt taaaagccac tgcttctaca tatatacaac gctgcgtggg cattaatcat
     7441 gttgaatgtt ttgcacattt ttttgataat ttgcaactaa taaattaaaa taacaataaa
     7501 aacctataat taatattggt tttctacaat tttaataaat taattttttt atatatctaa
     7561 aatgtaacca atttcttttg ttatcaccaa ataaacaacc acatgatgct gtttagagcc
     7621 tacggaagta cgggtagttt gttccagaac attctagtat tttcgtttat ttttgtttta
     7681 cctataaatt cagtgcatgg ctgctaaccg gcatcagtct tgaaagaaaa attgtgcaaa
     7741 aaacaccaag aattccaaac gggagaaagt taaaagtgtt ttaattttct taataaagaa
     7801 aagtctgaaa aaagaacatt aaaaaagtgt gtagttgttt aatttcaatt gcaaaaagaa
     7861 aaataattta aaaagtattt atttttgtga aattaatata aaacaaacac atcaaggcaa
     7921 gtttttatta aaaaaaataa aatgtagtaa caaaacgttt ttgttctttt gaaaaaagtg
     7981 tgatgtaaaa actcaggtaa tttgccagaa agaaaaacct tcaatatggt ttaagaataa
     8041 cgttactaag taattgtaaa ttgttctaga ttaaattttc tttggccatg acatttttag
     8101 aaaataaatc tagtatataa agaaaatttc accatgctaa taaaaagaaa tttctagcat
     8161 gcgatttttt aaagaaaatt tttcaaaatt ttttcgaaat tataatcgat atttcatgtt
     8221 ttcattcgtt aaaaagcata cccaatccac ccctaaaaaa ctagtaattc ttaacttaaa
     8281 gagatttcta gatttttatt aaaaattttc agtaccaatc ccactcccta cacttaacga
     8341 aaaacgtgcg tcattaaaat tatttatact tcatattttg attgattatt ttactaataa
     8401 tttgtatttt tctatttttt ttttattaaa cacagatgcc tgaagaagtt gaaacttttg
     8461 ctttccaagc tgaaattgct cagcttaaag gatccatgga ttctactgaa aatgttatca
     8521 agccttttat gcgttttaag gtacacatgg aaggttctgt caatggtcac gaatttgaaa
     8581 tcgaaggtga aggtgaaggt aaaccctacg aaggtactca aactgctaaa ttgcaagtta
     8641 ccaagggtgg tcccttgcca ttcgcctggg atattttgtc cccacaattc caatacggtt
     8701 ctaaggtata cgttaagcat cccgctgata ttcccgatta taaaaagttg tccttccccg
     8761 aaggttttaa gtgggaacgt gtaatgaatt tcgaagatgg tggtgttgtc accgttactc
     8821 aagattcctc tttgcaagat ggtactttta tttaccacgt caaattcatc ggtgtaaact
     8881 ttccatcaga tggtcctgtt atgcaaaaga aaaccttggg ttgggaacct tctactgaac
     8941 gtttgtatcc ccgtgatggt gtcttgaaag gtgaaattca taaggctttg aaattgaagg
     9001 gtggtggtca ctacttggtt gaatttaagt ccatctacat ggccaaaaag cccgttaagt
     9061 tgccaggtta ctactacgtc gattcaaaat tggatattac cagtcataat gaagattaca
     9121 ccgtagtaga acaatacgaa cgtgccgaag cccgtcacca cttgtttcaa taaaagcttg
     9181 cagccaaacc catcatcgac aaccatcaaa accaaaaaaa aaagacagaa gaagtgtgta
     9241 ttgaaaaaat tctcgaccta ttagctgaaa ctactcaact aaacattcaa tatacttttt
     9301 tttaaacaaa ctgttatttt aaaaatgata acgaatccaa aacaaaacac ttgtaaattt
     9361 tttttatttt caaatttgaa aaaaacatga atgaaaagaa caaattttaa agaaatgcac
     9421 tttaaaaaca cgaaaaaaaa acaagaacct aataaaatga aaggcttaag ctgcattgaa
     9481 tcataatatt ccaaaagaaa attttttaat gttttactac ttaatggggg gaaaaagagg
     9541 gaaaacaact aaaaatagta attgttaaaa tgcttggcaa aagtttaatg aacttgccga
     9601 ggtgtagagg aaactataaa aatgcatagt gagtgcatta aaaacagcac tagtttggcc
     9661 tcattttgat gggtacaaga ggaagacgaa caattttaaa aataactttc aagtggaaga
     9721 tagatttgct tgaaaacatt ttgagaattt actctagcta taaagataaa taacattact
     9781 tccktywtwc ccaagatttc tttaaactat agatgactca ttaatgaatc gttgcttttt
     9841 atttatgatt tccctcccct cttaaacaag tacagtttgt tttctatata catacctact
     9901 cagagttcta ttttttgtta gcaacagctc tgccaactgc ttgttgcctt ttaatagagt
     9961 tttgttgttg ctgtgttggt cttaaaatca tatgaagcat tttatgtggt gggttttata
    10021 actatttttg tttggatata gtatttttgt tttctgtact gtaactcaac aacacattgc
    10081 cttttatggt catataaatg agtaaatata ctatattgta tctaccacga tgatctcatt
    10141 cacatttttt agagtttaca ttggaaacaa atatttgaat tttgtaagaa aacactgccc
    10201 accaaatttt taagttgttt atttggatct ttgtgtaaaa gaggggtttt aagataaatt
    10261 tcaagttcat tttaatgtat catgggacgc taagatcaaa gaatatttgt tttaagtaat
    10321 gtaaagttat attttctgct tgatcaaatc tattataaga tcttatatca cacagtgaat
    10381 tagttcccaa gaaaggggta aagatgcata tcatttagct taaacgtttt tcataaaact
    10441 gaacgtcatg ggcaaatttt cattaaagta taattttctc caaagaactg gtctattgtc
    10501 ctcactattt tatatttcca tagtactgga caggaacgcg gtttaaggat ttgcgttttt
    10561 agttaatata aatcaaagtt tttgactaca ccagcccgca ctgacggaca caccgaagcc
    10621 ccggcggcaa ccctcagcgg atgccccggg gcttcacgtt ttcccaggtc agaagcggtt
    10681 ttcgggagta gtgccccaac tggggtaacc tttgagttct ctcagttggg ggcgtagggt
    10741 cgccgacatg acacaagggg ttgtgaccgg ggtggacacg tacgcgggtg cttacgaccg
    10801 tcagtcgcgc gagcgcgact agtacaagcc gaattctgca gat
//
